Coding

Part:BBa_K174000:Design

Designed by: The Newcastle 2009 iGEM team   Group: iGEM09_Newcastle   (2009-09-26)

The sequence is taken from E. coli strain DH5 alpha. Genbank accession number for E. coli MG1655 strain is NC_000913.2

Construction

Plasmid part BBa_J04450 (pSB1AT3) and our construct were treated with EcoRI and SpeI

Newcastle 2009 sspB 1.png

The backbone and the sspB insert were then ligated

Newcastle 2009 sspB 2.png